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膜蛋白纯化试剂盒
Membrane Protein Purification Kit
Membrane Protein Purification Kit
| Bead type | Mag Sepharose Magnetic Beads |
| Binding Capacity | approximately 50 mg histidine-tagged protein/ml medium (~500 μg/purification run) |
| Critical Micelle Concentrations (CMC) | DDM: 0.009% (0.17 mM) DM: 0.09% (1.8 mM) LDAO: 0.02% (1-2 mM) FOS12: 0.05% (1.5 mM) C12E8: 0.005% (0.09 mM) Cymal 5: 0.12% (2-5 mM) OG: 0.53% (18-20 mM) |
| Detergent Class | DDM: Non-ionic DM: Non-ionic LDAO: Zwitter-ionic FOS12: Non-ionic C12E8:Non-ionic Cymal 5:Zwitter-ionic OG:Non-ionic |
| Kit contents | His Mag Sepharose Ni, 3 x 1 ml vial n-Dodecyl-b-D-maltoside (DDM) ,10% (w/v) aqueous solution, 1 ml n-Decyl-b-D-maltoside (DM), 10% (w/v) aqueous solution, 1 ml Lauryldimethylamine-N-oxide (LDAO), 10% (w/v) aqueous solution, 1 ml n-Dodecylphosphocholine (FOS12), 10% (w/v) aqueous solution, 1 ml Dodecyl octaethyleneglycol ether (C12E8), 10% (w/v) aqueous solution, 1 ml Cyclohexyl-1-pentyl-b-D-maltoside (Cymal 5), 10% (w/v) aqueous solution, 1 ml n-Octyl-b-D-glucoside (OG), 20% (w/v) aqueous solution, 1 ml Phosphate buffer (stock), pH 7.4 (160 mM sodium phosphate, 4 M NaCl), 100 ml 2 M imidazole pH 7.4, 100 ml |
| Matrix | Highly cross-linked spherical agarose (Sepharose) including magnetite |
| Metal Ion Capacity | ~21 μmol Ni²⁺/ml medium |
| Number of Runs | Kit is sufficient for two complete screening experiments |
| Operating Temp. | Ambient |
| Storage Solution | 20% ethanol, 5% medium slurry |
| Storage conditions | +2°C to +8°C |
产品详情
- 可简化带组氨酸标签的膜蛋白的去污剂筛选。
- 可同时对溶解和纯化效率进行结果分析。
- 便捷的磁珠规格实现快速简便筛选。
- 即用型去污剂溶液。
- 简单易操作的方案。
膜蛋白纯化试剂盒
膜蛋白纯化试剂盒设计用于带组氨酸标签的膜蛋白的高效去垢剂筛选。将细胞膜的小份等分试样溶解在不同的去垢剂中，然后使用 His Mag Sepharose Ni 快速纯化。使用相同的去污剂溶解后直接进行纯化步骤。可通过多种方法（如 Western 印迹、凝胶过滤或光散射）进行结果分析。膜蛋白纯化试剂盒足以根据所包括的方案进行两次完整的筛选实验。
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