PreDictor™ RoboColumn™ Ni Sepharose™ 6 Fast Flow

Prepacked minicolumns with Ni Sepharose™ 6 Fast Flow resin for HTPD of purification of his-tagged proteins.

These PreDictor™ RoboColumn™ minicolumns for high-throughput process development (HTPD) are prepacked with Ni Sepharose™ 6 Fast Flow resin for purification of histidine-tagged (his-tagged) proteins.

  • Supports high-throughput process development (HTPD) — enables parallel screening of chromatographic conditions using a miniaturized column format and robotic workstation.
  • Miniaturized column format — facilitates screening of a wide range of parameters with small sample consumption.
  • Reduced experimental time — automation and parallel screening minimize labor and save time.
  • Increased process understanding — high-throughput workflow allows investigation of enlarged experimental workspace for better process understanding.
  • Packed with Ni Sepharose™ 6 Fast Flow resin — for purification of his-tagged proteins.

Ni Sepharose™ 6 Fast Flow IMAC resin

Ni Sepharose™ 6 Fast Flow immobilized metal affinity chromatography (IMAC) resin is designed for purification of his-tagged proteins. It is based on cross-linked agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.

Ni Sepharose™ 6 Fast Flow resin meets industrial demands with security of supply, robust performance, and regulatory support.

Learn more about Ni Sepharose™ Fast Flow resin.

PreDictor™ RoboColumn™ units for HTPD

PreDictor™ RoboColumn™ units are prepacked, miniaturized columns that support HTPD using a robotic liquid handling workstation for fully automated and parallel chromatographic separations. HTPD work can be performed using PreDictor™ RoboColumn™ units alone, or as a complement to PreDictor™ 96-well filter plates. PreDictor™ RoboColumn™ units can be operated in true chromatography mode, enabling dynamic binding capacity studies, while 96-well plates enable the study of static binding capacities.

PreDictor™ RoboColumn™ units are supplied in rows of eight columns with the same chromatography resin, and can be individually and easily arranged on an array plate containing 96 positions.

Read more about HTPD.

* RoboColumn is a trademark of Repligen GmbH.

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