Deaza dGTP

Nucleotide analog (candidate substrate) for processive telomerase, to improve PCR yields and make sequencing of GC rich sequences easier.

物理状态	液体
浓度	5 mM
颜色	Colorless
许可	For licensing information, see the Licensing Statements page in the About Us section.
包装规格	2 μmoL

SKU： 27209002 分类：实验室耗材, 蛋白质印迹和其他基因组学试剂

Deaza dGTP is a nucleotide analog that is a candidate substrate for processive telomerase. Its use can improve Polymerase Chain Reaction (PCR) yields and makes sequencing of GC rich sequences easier.

For reducing band compressions* in sequencing gels.
Supplied as convenient solutions at pH 7.0 and 7.5. c⁷dGTP is supplied in purified water as 5 mM and 10 mM solutions.

Deaza dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase. It can also be applied to dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA. Partial substitution of Deaza dGTP for dGTP in PCR can improve the product yield when the template is GC-rich.

Technical Information:

*Band compressions are ambiguous band patterns on DNA sequencing gels. This is caused by stable, intra-strand secondary structures which are not fully denatured during electrophoresis. The likelihood of occurrence can be reduced through substitution of nucleotide analogs within sequencing mixers. To resolve G-band compressions, use 7-deaza dGTP (c⁷dGTP).