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Whatman Protran BA membranes

Nitrocellulose membranes for use in flow-through and blotting applications.

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Nitrocellulose membranes for use in flow-through and blotting applications, providing a small pore size for low nonspecific binding and greater sensitivity of small proteins.

  • Range includes BA79, BA83 and BA85
  • Manufactured for vertical-flow assays: Removes problems caused by capillary rise.
  • Small pore structure: Accurate results; low nonspecific binding; greater sensitivity
  • One hundred percent pure nitrocellulose: Provides high binding capacity
  • Autoclavable

For flow-through and Western blotting, Southern blotting and Northern blotting assays, small-pore unsupported membranes are ideal as they are highly sensitive with large surface area and high protein binding capacity. Choosing a membrane with a small pore size avoids the small protein migrating too deeply into the membrane surface before binding into the nitrocellulose which causes a loss of signal or sensitivity.

Flow-through immunoassays offer a lower degree of integration than lateral-flow immunoassays which makes development and production easier. It may also be easier to develop multi-analyte tests because the spatial position of a capture molecule on the membrane is not linked to the assay dynamics as in a lateral flow assay.

Line blot assays are an easy to develop, lab-based assay that can be multiplexed to support limited sample volumes. Confirmatory blots are usually Western blots (e.g., chasing anti-HIV antibodies in patient sera). Southern blot analysis is especially useful for analysis of repetitive sequences because multiple similar sequences in the genome can be analyzed by a single probe.

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