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Deaza dGTP

Nucleotide analog (candidate substrate) for processive telomerase, to improve PCR yields and make sequencing of GC rich sequences easier.

7-脱氮-2′-脱氧鸟苷-5′-三磷酸,5 mM 溶液 (c⁷dGTP)
物理状态 液体
浓度 5 mM
颜色 Colorless
许可 For licensing information, see the Licensing Statements page in the About Us section.
包装规格 2 μmoL

Deaza dGTP is a nucleotide analog that is a candidate substrate for processive telomerase. Its use can improve Polymerase Chain Reaction (PCR) yields and makes sequencing of GC rich sequences easier.

  • For reducing band compressions* in sequencing gels.
  • Supplied as convenient solutions at pH 7.0 and 7.5. c⁷dGTP is supplied in purified water as 5 mM and 10 mM solutions.

Deaza dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase. It can also be applied to dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA. Partial substitution of Deaza dGTP for dGTP in PCR can improve the product yield when the template is GC-rich.

Technical Information:

*Band compressions are ambiguous band patterns on DNA sequencing gels. This is caused by stable, intra-strand secondary structures which are not fully denatured during electrophoresis. The likelihood of occurrence can be reduced through substitution of nucleotide analogs within sequencing mixers. To resolve G-band compressions, use 7-deaza dGTP (c⁷dGTP).

重组胶原蛋白CMC平台搭建

主讲嘉宾:周玥职位:Cytiva资深行业拓展专家

课程分类:线上应用课程, 线上专题课程 – 重组蛋白基础课堂

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