AcroPrep 24-well filter plates with Omega membrane

For ultrafiltration applications including desalting and diafiltration

AcroPrep™ 24-well filter plates with Omega™ membrane, a Pall Life Sciences product, can be used for ultrafiltration applications including desalting, diafiltration, concentration or fractionation of protein and nucleic acid samples.

The Omega ultrafiltration membrane is a polyethersulfone (PES) membrane specifically modified to minimize protein and nucleic acid binding. The Omega membrane works on the premise of size exclusion, where samples are fractionated by molecular weight. Larger molecules can be separated from smaller molecules by selecting the appropriate molecular weight cut-off (MWCO) and filtering the sample through these plates by either centrifugation, vacuum, or positive pressure. Typical results are ≥ 90% recovery of target molecules.

The 24-well filter plates are available with a variety of MWCOs, ranging from 1 kDa to 100 Kda, to use with a wide range of biomolecules. Receiver plates are supplied with the filter plates to ensure the easy collection of the filtrate.

Applications

Concentration and purification of peptides, proteins, oligonucleotides, DNA, and RNA
Clean-up of labelling and PCR reactions
Desalting and buffer exchange
Fractionation based on size exclusion

AcroPrep 24-Well Filter Plate Features

Receiver plate and lid are included
Intrinsic plate and membrane properties minimize sample loss from non-specific binding
Automation friendly – compatible with all major automation platforms
Designed in accordance with the ANSI/SLAS X-2004 standards
Vacuum compatible – Compatible with all popular vacuum and positive pressure manifolds
Centrifugation – Suitable for centrifugation with compatible rotors

Specifications

Filter Media

Omega (modified polyethersulfone membrane)

Plate Housing

Polypropylene

Lid

Polystyrene

Dimensions

Length: 12.8 cm (5.0 in.) Width: 8.6 cm (3.4 in.) Height (With receiver plate): 73.9 mm (2.9 in.) Height (Without receiver plate): 37.4 mm (1.5 in.) Well-Bottom Area: 1.6 cm2 (0.24 in.2)

Recommended Working Volume

7 mL for vacuum 6 mL for centrifugation

Recommended Operating Vacuum

38 cm Hg (15 in. Hg)

Recommended Centrifugal Force

1,500 x g

Recommended Positive Pressure

50 psi

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