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HiTrap™ Capto™ Octyl column is prepacked with a HIC resin for small scale purification
- Prepacked with Capto™ Octyl high-flow and high-capacity HIC resin for capture and intermediate purification processes
- Lab-scale column format, suitable for screening of selectivity, binding and elution conditions, and for small-scale purifications in research and process development
- Convenient HiTrap™ format for easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system
About HiTrap™ column format
HiTrap™ columns are manufactured from biocompatible polypropylene that does not interact with biomolecules. The column formats are well-suited for preparative purifications as well as for screening and optimization of purification conditions when developing a new method.
When needed, two columns can easily be connected in series to increase the bed height.
Capto™ Octyl – a high-flow and high-capacity HIC resin
Capto™ Octyl hydrophobic interaction chromatography resin has an octyl ligand for the capture and intermediate purification of monoclonal antibodies (mAbs), mAb variants, and other biomolecules. It combines high capacity, narrow specification range, high flow rate, and low backpressure to meet the demands of large-scale biopharmaceutical manufacturing.
Not sure which HIC resin you should use? We recommend starting with one of the kits below:
- PreDictor™ Capto™ HIC screening kit, a set of 96-well plates prefilled with six Capto™ HIC resins for use in high-throughput process development (HTPD)
- HiTrap™ Capto™ HIC selection kit, a set of five Capto™ HIC resins in HiTrap™ columns for resin selection and condition screening
Capto™ Octyl is available in other formats (Figure 1):
- Bulk resin bottles and containers of Capto™ Octyl resin for column packing
- PreDictor™ Capto™ Octyl RoboColumn™ units, miniaturized columns for HTPD
- PreDictor™ Capto™ Octyl multiwell plates, 96-well plates for high-throughput screening or process development
- HiScreen™ Capto™ Octyl columns optimized for method and process development
- ReadyToProcess™ Capto™ Octyl columns, in various sizes for scale-up and GMP manufacturing
Fig 1. Prepacked chromatography formats available from Cytiva to support various scales, from screening to GMP manufacturing.
